OligoArchitect™ Primer and Probe Design

Oligo Search Range

Sense Primer Search Range	:	To:
Anti-Sense Primer Search Range	:	To:
Amplicon Search Range	:	To:

Advanced Primer Parameters

Hairpin Maximum ΔG (3' End)				:	-kcal/mol
Hairpin Maximum ΔG (Internal)				:	-kcal/mol
3' End Maximum ΔG				:	-Kcal/mol
Self Dimer Maximum ΔG (3' End)				:	-kcal/mol
Self Dimer Maximum ΔG (Internal)				:	-kcal/mol
Run/Repeat (dinucleotide) maximum length				:	bp
GC Clamp - Consecutive G/C's at 3' End				:
	GC%	:	To

Advanced Primer Pair Parameters

Maximum Primer Pair Tm Mismatch	:	°C
Cross Dimer Maximum ΔG (3'End)	:	-kcal/mol
Cross Dimer Maximum ΔG (Internal)	:	-kcal/mol

Reaction Conditions

Primer Concentration	:	nM
Monovalent Ion Concentration	:	mM
Free Mg⁺⁺ Ion Concentration	:	mM
Total Na[+] Equivalent	:	mM
Temperature for Free Energy Calculation	:	°C